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    Laboratory Services Section
    MC 1947
    PO Box 149347 Austin, TX 78714-9347
    1100 W. 49th Street
    Austin, TX 78756-3199

    Phone: (512) 776-7318
    Fax: (512) 776-7294

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EHEC EIA for the detection of Shigatoxin acceptance-rejection criteria

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State of Texas Seal

TEXAS DEPARTMENT OF STATE HEALTH SERVICES

DAVID L. LAKEY, M.D.
COMMISSIONER

1100 W. 49th Street • Austin, Texas 78756
P.O. Box 149347 • Austin, TX 78714-9347
1-888-963-7111 • http://www.dshs.state.tx.us
TDD: 1-800-735-2989

 

 

   

November 1, 2008

 

Dear Submitter:

Re: EHEC EIA for the detection of Shigatoxin acceptance/rejection criteria

Effective December 1, 2008 the Texas Department of State Health Services (DSHS), Laboratory Services Section will be enforcing specimen acceptance/rejection criteria for clinical specimens for the EHEC (E. coli Shiga toxin) test.

Test

Specimen type

Time from collection to arrival at the laboratory

Temperature

Shipping requirements

EHEC EIA for the detection of Shigatoxin

Raw stool
Broth (MacConkey’s broth or GN broth)

Up to 7 days

Cold 2° to 8°C

Ship on cold packs

Raw stool
Broth (MacConkey’s broth or GN broth)

Greater than 7 days

Frozen, -70°C or lower

Ship overnight on dry ice.

Cary-Blair Based transport medium

Up to 3 days

Cold 2° to 8°C

Ship on cold packs

Cary-Blair Based transport medium

Greater than 3 days

Frozen, -70°C or lower

Ship overnight on dry ice.

Transfer from Raw stool or broth to fresh broth made prior to 7 days

Up to 7 days from date of transfer.
Transfer must be clearly marked with date of transfer. Submit both the original specimen and the transfer. Note: Toxin expression may be lost over time or upon serial passage.

Cold 2° to 8°C

Ship on cold packs

Any specimens that do not meet these criteria at the date of receipt in our laboratory will be considered unsatisfactory for testing and will be immediately discarded.  Although not intended to disrupt vital public health services to our programs and citizens of Texas, these guidelines will ensure the quality of test results and compliance with manufacturers’ specimen acceptance criteria.  General submission guidelines can be found on our website: http://www.dshs.state.tx.us/lab/MRS_intro.shtm

  • Please distribute this letter to all staff responsible for submitting samples
  • Please do not ship specimens to the DSHS laboratory on Friday or the day before a federally observed holiday.

If we can be of any assistance to you in interpreting these guidelines, please contact Tamara Baldwin at 1-888-963-7111 ext. 7582. Thank you for your continued support.

Sincerely,




Susan U. Neill, PhD, MBA
Director, Laboratory Services Section

Last updated May 19, 2010