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    Laboratory Services Section
    MC 1947
    PO Box 149347 Austin, TX 78714-9347
    1100 W. 49th Street
    Austin, TX 78756-3199

    Phone: (512) 776-7318
    Fax: (512) 776-7294

    Phone Us Toll Free at:
    (888) 963-7111, ext. 7318

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Microbiology Laboratory Tests: Malaria to Mosquitoes

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Microbiology
Malaria (Serological – Forwarded by TDSHS to CDC for testing.)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  3 weeks Reference Range:  Nonreactive
Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.

Specimen Requirements

Specimen Collection: Venipuncture Sample Type: Serum
Volume/Amount Required: 10 mL whole blood Preferred Specimen:  Single Serum
Collection/Preservation: Red top or tiger top tube Storage Instructions: Do not freeze or refrigerate whole blood. Separated serum may be held at 2-8°C
Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing Sample Container: Red top or tiger top tube
Sample Test Kit: Availability:
Diagnostic Information:  If blood films are negative but evidence for malaria is strong then serum is sent to the CDC.  Serology is by prior arrangement only, (512) 458-7760.  Once cleared, serum specimens are sent to the CDC.  Antibody may persist for years, so that an elevated titer cannot be used as evidence for current infection.  Emphasis is placed on blood films. A detailed patient history is required.

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature:  Ambient temperature for specimens on the blood clot, separated serum at 2-8°C (refrigerated) or -20°C (frozen). Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code: 86750 Fees:


Microbiology
Malaria (Microscopic – Direct Stain Giemsa)
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Test Includes: Microscopic examination

Reporting

Results Available: 1 day Contact #s: (512) 458-7560

Reference

Method: Microscopic examination  
Turnaround Time:  24 hours Reference Range:  No parasites found
Limitations:  Anticoagulants such as EDTA in venous blood specimens can interfere with parasite morphology and staining characteristics Interpretation:  No parasites found indicates that there were no visible parasites in the specimens submitted.

Specimen Requirements

Specimen Collection: Finger stick or Venipuncture Sample Type: Thick/thin Blood Smears; Blood in Purple top
Volume/Amount Required: 2 mL whole blood Preferred Specimen:  Thick/thin Blood Smears; Blood in Purple top
Collection/Preservation: Collect blood by finger stick. If blood is collected by venipuncture in purple top tube, make 2 thick and 2 thin smears on glass slides within one hour of collection. Storage Instructions: ambient temperature
Causes for Rejection: Incorrect blood tube used. Sample Container: Purple top vacutainer
Sample Test Kit: Availability: Monday-Friday
With prior approval on Saturday/Sunday
Diagnostic Information:  Anticoagulants such as EDTA in venous blood specimens can interfere with parasite morphology and staining characteristics, this can be further compounded by excessive delay prior to making smears. In such cases capillary blood samples are preferable (finger sticks) . If venous blood is used at least 2 thin and 2 thick smears should be made within 1 hour.

Specimen Submission

Required Request Form: G-2B Specimen Handling: Handle body fluids using universal precautions.
Transport Temperature:  Ambient temperature Shipping Requirements: Triple contained in accordance with federal shipping regulations for diagnostic specimens.

Billing

CPT Code: 87207 Fees:


Microbiology

Melioidosis Culture (Isolation) See also Aerobic Bacterial Culture (Isolation)
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Test Includes: Conventional biochemicals

Reporting

Results Available: 7-21 days

Contact #s: (512) 458-7582

Reference

Method: Conventional biochemicals

 

Turnaround Time:  7-21 days

Reference Range:  No bacteria isolated.

Limitations:

Interpretation: Isolation of Burholderia pseudomallei should always be considered significant.  It is a select agent and must be reported following guidelines for select agents. 

Specimen Requirements

Specimen Collection: Venipuncture, biopsy, needle aspiration

Sample Type: blood, tissue, aspirates

Volume/Amount Required: 20 mL whole blood, small piece of tissue,

Preferred Specimen: blood, tissue, aspirates

Collection/Preservation: Standard collection, transport, and storage techniques are sufficient to ensure the recovery of this organism.

Storage Instructions: ambient temperature

Causes for Rejection: Incorrect collection tube, name on tube/form does not match; broken in transport.

Sample Container: Tiger top or red top vacutainer;  sterile, leak-proof container.

Sample Test Kit:

Availability: Monday-Friday

Diagnostic Information:  B. pseudomallei is the etiologic agent of melioidosis.  Mortality in patients with fulminant sepsis approaches 90%,  Melioidosis is most prevalent in Southeast Asia and northern Australia.  This organism should be considered in any individual with tuberculosis-like disease who has a travel history to a region of endemicity, even if travel preceded the illness by decades.

Specimen Submission

Required Request Form: G-2B

Specimen Handling: Handle body fluids using universal precautions.

Transport Temperature:   Ambient temperature.

Shipping Requirements: Triple contained in accordance with federal shipping regulations for diagnostic specimens.

Billing

CPT Code: 87040

Fees:

Microbiology
Melioidosis (Culture – Identification) See also Aerobic Bacterial Culture,  (Identification)
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Test Includes: Conventional Biochemicals, PCR

Reporting

Results Available: PCR 1 day; 4-21 days

Contact #s: (512) 458-7582/ 458-7185

Reference

Method: Conventional biochemicals, PCR

 

Turnaround Time:  PCR 1 day; PCR 4-21 days

Reference Range:  Genus and species reported.

Limitations:

Interpretation: Identification of Burkholderia pseudomallei should always be considered significant. It must be reported following guidelines for a select agent.

Specimen Requirements

Specimen Collection:

Sample Type: Pure culture

Volume/Amount Required: one specimen per patient

Preferred Specimen:  Pure culture on agar slant

Collection/Preservation: Ambient temperature

Storage Instructions: Ambient temperature

Causes for Rejection: Expired transport media, broken or compromised in transport.

Sample Container: Agar slant in screw cap tube

Sample Test Kit:

Availability: Monday-Friday
With prior notification, Saturday-Sunday

Diagnostic Information:  B. pseudomallei is the etiologic agent of melioidosis.  Mortality in patients with fulminant sepsis approaches 90%,  Melioidosis is most prevalent in Southeast Asia and northern Australia.  This organism should be considered in any individual with tuberculosis-like disease who has a travel history to a region of endemicity, even if travel preceded the illness by decades.

Specimen Submission

Required Request Form: G2-B

Specimen Handling: Infectious agent, Biosafety level 2

Transport Temperature:  Ambient temperature

Shipping Requirements: Shipping Requirements: Triple contained in accordance with federal shipping regulations for infectious agents.

Billing

CPT Code: 87077

Fees:

 

Microbiology
Meningitis, Meningococcal Culture (Isolation)
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Test Includes: Conventional biochemicals, Serological typing

Reporting

Results Available: Preliminary 1day;
Final report 5-10 days
Contact #s: (512) 458-7582

Reference

Method: Conventional Biochemicals, serotype determined by slide agglutination
Turnaround Time:  5-10 days Reference Range:  No bacteria isolated
Limitations: Specimen should be collected prior to initiation of antibiotic therapy Interpretation:

Specimen Requirements

Specimen Collection: Venipuncture, Spinal tap Sample Type:
Volume/Amount Required: 10 mL whole blood, 1-2 mL spinal fluid Preferred Specimen:  Blood; Spinal Fluid
Collection/Preservation: Collect blood by venipuncture in a red top or tiger top vacutainer. Store at ambient temperature. Inocuation into an aerobic blood culture bottle is also acceptable. Collect CSF by spinal tap. Transport at 2-8° C Storage Instructions: Store at 2-8° C
Causes for Rejection: Incorrect collection tube used. Sample Container: Red or tiger top vacutainer
Sample Test Kit: Availability: Monday-Friday
Diagnostic Information:  See Aerobic Bacterial Culture (Isolation); Isolation of N. meningitidis will be serogrouped and the report will be called within 24 hours of isolation.Molecular typing on isolates at TDSHS upon request. 

Specimen Submission

Required Request Form: G-2B Specimen Handling: Handle body fluids using universal precautions.
Transport Temperature:  2-8° C Shipping Requirements:   Triple contained in accordance with federal shipping regulations for diagnostic specimens.

Billing

CPT Code: 87070 Fees:


Microbiology
Meningitis, Meningococcal Culture (Identification)
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Test Includes: Conventional biochemicals, Serological typing

Reporting

Results Available: Preliminary 1day;
Final report 5-10 days
Contact #s: (512) 458-7582

Reference

Method: Conventional Biochemicals, serotype determined by slide agglutination
Turnaround Time:  5-10 days Reference Range:  By report
Limitations: Organism must be viable for culture studies. Interpretation: By report

Specimen Requirements

Specimen Collection: Sample Type: Pure culture
Volume/Amount Required: One specimen per patient Preferred Specimen:  Pure culture on blood agar
Collection/Preservation: Ambient temperature, CO2 environment. Storage Instructions: Ambient temperature; Do not refrigerate
Causes for Rejection: Name on tube/form do not match; broken in transport Sample Container:
Sample Test Kit: Availability: Monday-Friday
With prior notification: Saturday-Sunday
Diagnostic Information:  Pure cultures submitted on Blood Agar slants can be serogrouped upon arrival. Preliminary reports will be called within 24 hours of receipt. See Aerobic Bacterial Culture, (Identification) Molecular typing at TDSHS upon request. Molecular typing is routinely performed on identified outbreak isolates.

Specimen Submission

Required Request Form: G-2B Specimen Handling: Infectious agent, Biosafety level 2.
Transport Temperature:  Ambient temperature Shipping Requirements: Triple contained in accordance with federal shipping regulations for infectious agents.

Billing

CPT Code: 87077 Fees:


Microbiology
Meningitis, Meningococcal Culture (Grouping)
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Test Includes: slide agglutination

Reporting

Results Available: Preliminary 1day;
Final report 3-5 days
Contact #s: (512) 458-7582

Reference

Method: slide agglutination  
Turnaround Time:  3-7 days   Reference Range:  By report
Limitations: Organism must be viable for serological typing. Interpretation: By report

Specimen Requirements

Specimen Collection: Sample Type: Pure culture
Volume/Amount Required: one specimen per patient. Preferred Specimen:  Pure culture on blood agar
Collection/Preservation: Ambient temperature, CO2 environment. Storage Instructions: ambient temperature, do not refrigerate
Causes for Rejection: name on tube/form do not match; broken in transport Sample Container: Agar slant in screw cap tube
Sample Test Kit: Availability: Monday-Friday
With prior notification: Saturday-Sunday
Diagnostic Information:  Serogrouping performed on all isolates and referred cultures. Pure cultures submitted on Blood Agar slants can be serogrouped upon arrival. Preliminary reports will be called within 24 hours of receipt.

Specimen Submission

Required Request Form: G-2B Specimen Handling: Infectious agent, biosafety level 2
Transport Temperature:  Ambient  temperature Shipping Requirements: Triple contained in accordance with federal shipping regulations for infectious agents.

Billing

CPT Code: 87077 Fees:


Microbiology
Microsporidiosis (Microscopic – Modified Trichrome Stain)
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Test Includes: Microscopic examination

Reporting

Results Available: 3 days Contact #s: (512) 458-7560

Reference

Method: Microscopic examination  
Turnaround Time:  3 days Reference Range:  No parasites found
Limitations:  Interpretation:

Specimen Requirements

Specimen Collection: feces, tissue Sample Type: feces, tissue
Volume/Amount Required: 15 mL liquid stool, 15 g stool Preferred Specimen:  Feces; tissue
Collection/Preservation: Collect stool in clean, dry container. Immediately transfer to formalin preservative. Storage Instructions: Ambient temperature
Causes for Rejection:  Unpreserved stool > 5 hours old. Sample Container: Formalin transport for parasites
Sample Test Kit: Availability:  Monday-Friday
Diagnostic Information:  Feces should be formalin preserved. Other suspect tissue-send tissue or stained slide.

Specimen Submission

Required Request Form: G-2B Specimen Handling:  Handle body fluids using universal precautions. Formalin is a poison, handle with care.
Transport Temperature:  Ambient temperature Shipping Requirements: Triple contained in accordance with federal shipping regulations for diagnostic specimens.

Billing

CPT Code: 87209 Fees:


Microbiology
Milk, Quality Check (Culture- SPC)
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Test Includes: Standard plate count

Reporting

Results Available: 4 days Contact #s: (512) 458-7562

Reference

Method: Standard plate count  
Turnaround Time:  4 days Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Milk Sample Type: Milk
Volume/Amount Required: According to requirements of the State Milk Program Preferred Specimen:  Milk
Collection/Preservation: Milk is collected by state health officials Storage Instructions:  0-4.4° C
Causes for Rejection: Temperature control out of range Sample Container: whirlpak or milk container
Sample Test Kit: Availability: With prior notification or as routinely scheduled
Diagnostic Information:  Samples are accepted only from state health officials. Prior notification by the collecting authority is required.

Specimen Submission

Required Request Form: G-84 or G-21 Specimen Handling: According to FDA guidelines
Transport Temperature:  0-4.4° C Shipping Requirements: Leak-proof containers, refrigerated

Billing

CPT Code: Fees:


Microbiology
Milk, Quality Check (Inhibitors)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  4 days Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Milk
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Samples are accepted only from state health officials.  Prior notification by the collecting authority is required.

Specimen Submission

Required Request Form: G-84 or G-21 Specimen Handling: According to FDA Guidelines
Transport Temperature:  0-4.4º C   Shipping Requirements: Leak-proof containers, refrigerated

Billing

CPT Code: Fees:


Microbiology
Milk, Quality Check (Phosphatase)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  4 days Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Milk
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Samples are accepted only from state health officials.  Prior notification by the collecting authority is required.

Specimen Submission

Required Request Form: G-84 or G-21 Specimen Handling: According to FDA Guidelines
Transport Temperature:  0-4.4º C Shipping Requirements: Leak-proof containers, refrigerated

Billing

CPT Code: Fees:


Microbiology
Milk, Quality Check (Coliforms)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  4 days Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Milk
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Samples are accepted only from state health officials.  Prior notification by the collecting authority is required. 

Specimen Submission

Required Request Form: G-84 or G-21 Specimen Handling: According to FDA Guidelines
Transport Temperature:  0-4.4º C Shipping Requirements: Leak-proof containers, refrigerated

Billing

CPT Code: Fees:


Microbiology
Milk, Quality Check (Added Water)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  4 days Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Milk
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Samples are accepted only from state health officials.  Prior notification by the collecting authority is required.

Specimen Submission

Required Request Form: G-84 or G-21 Specimen Handling: According to FDA guidelines
Transport Temperature:  0-4.4º C   Shipping Requirements: Leak-proof containers, refrigerated.

Billing

CPT Code: Fees:


Microbiology
Milk, Quality Check (Aflatoxin)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  4 days Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Milk
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Samples are accepted only from state health officials.  Prior notification by the collecting authority is required.

Specimen Submission

Required Request Form: G-84 or G-21 Specimen Handling: According to FDA guidelines
Transport Temperature:  0-4.4º C Shipping Requirements: Leak-proof containers, refrigerated.

Billing

CPT Code: Fees:


Microbiology
Milk, Quality Check (Somatic cell counts)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  4 days Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Milk
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Samples are accepted only from state health officials.  Prior notification by the collecting authority is required.

Specimen Submission

Required Request Form: G-84 or G-21 Specimen Handling: According to FDA guidelines
Transport Temperature:  0-4.4º C Shipping Requirements: Leak-proof containers, refrigerated.

Billing

CPT Code: Fees:


Microbiology
Milk, Quality Check (Pathogens)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  14 days Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Milk
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Samples are accepted only from state health officials.  Prior notification by the collecting authority is required.

Specimen Submission

Required Request Form: G-84 or G-21 Specimen Handling: According to FDA guidelines
Transport Temperature:  0-4.4º C Shipping Requirements: Leak-proof containers, refrigerated.

Billing

CPT Code: Fees:


Microbiology
Molecular typing (PFGE)
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Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  14 days   Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Pure culture, safely contained
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Cultures for testing are accepted with prior notification, please call (512) 458-7214.  PFGE testing is a useful tool for determining strain relateness and possible l outbreaks or nosocomial outbreaks.  At least two isolates from different individuals must be submitted in a suspected outbreak.  As part of the PulseNet program, all E. coli O157:H7 and Listeria , selected Salmonella, Shigella, and Campylobacter, and Vibrio, Yersinia are molecularly typed.  The patterns are posted to the National PulseNet Database allowing for identification of outbreaks that may not be regionally contained.

Specimen Submission

Required Request Form: G-2B Specimen Handling:
Transport Temperature:  Ambient (Room) temperature Shipping Requirements:

Billing

CPT Code: 87152 Fees:


Microbiology
Mosquitoes (Identification only)
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Test Includes:

Reporting

Results Available: Contact #s: 512-458-7615

Reference

Method:  
Turnaround Time:  3-5 days Reference Range: By report.
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Live Mosquitoes
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Mosquitoes are accepted only from health officials who have been trained in the Texas Department of State Health Services state surveillance program.

Specimen Submission

Required Request Form: G-14 Specimen Handling:
Transport Temperature:  Wet Ice (Cold packs) Shipping Requirements:

Billing

CPT Code: Fees:


Microbiology
Mosquitoes (Culture – Virus Isolation/Detection and Identification)
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Test Includes:

Reporting

Results Available: Contact #s: 512-458-7615

Reference

Method:  
Turnaround Time:  5-14 days Reference Range: No arbovirus isolated.
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Live Mosquitoes
Collection/Preservation: Storage Instructions:
Causes for Rejection: Sample Container:
Sample Test Kit: Availability:
Diagnostic Information:  Mosquitoes are accepted only from health officials who have been trained in the Texas Department of State Health Services state surveillance program. Specimens are tested for the presence of arboviruses using cell cultures for isolation.

Specimen Submission

Required Request Form: G-14 Specimen Handling:
Transport Temperature:  Wet Ice (Cold Packs) Shipping Requirements:

Billing

CPT Code: Fees:


Last updated June 06, 2013