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    Laboratory Services Section
    MC 1947
    PO Box 149347 Austin, TX 78714-9347
    1100 W. 49th Street
    Austin, TX 78756-3199

    Phone: (512) 776-7318
    Fax: (512) 776-7294

    Phone Us Toll Free at:
    (888) 963-7111, ext. 7318

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Microbiology Laboratory Tests: Mumps to Mycoplasmosis

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Microbiology
Mumps (Culture - Isolation)
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Mumps (Culture - Isolation)
Test Includes: Cell culture

Reporting

Results Available: 2-14 days Contact #s: 512-458-7594

Reference

Method: Cell Culture  
Turnaround Time:  2-14 days Reference Range:  No virus isolated
Limitations: Interpretation: A result of “No virus isolated” does not necessarily mean absence of a viral agent.  The success of virus isolation depends a great deal on the submission of the proper specimen, collected at the right time, adequately maintained, and shipped with the least possible delay.

Specimen Requirements

Specimen Collection: Specimens should be collected at an appropriate anatomic site and at the proper time after infection because viruses are generally shed for only a short period of time.  Refer to Specimen Collection by Type table for additional instructions.  Sample Type: See preferred specimen.
Volume/Amount Required: 2-5 mL of CSF, 10-20 mL of urine, or saliva collected with swab in 2-3 mL of viral transport media. Preferred Specimen:  CSF; Urine; Saliva
Collection/Preservation: Viral transport media. Storage Instructions: Arriving < 48 hours after collection, store and send at 2-8° C. Arriving > 48 hours after collection, store and send at -70° C (dry ice).
Causes for Rejection: Specimens submitted on preservative such as formalin. Sample Container: Sterile container
Sample Test Kit: Availability: Monday - Friday
Diagnostic Information:  Specimens are inoculated onto a variety of cell cultures.  If characteristic CPE or hemadsorption is observed, confirmation of identification will be performed.

Specimen Submission

Required Request Form: G-2V Specimen Handling:
Transport Temperature:  
Arriving <48 hours:  2-8 °C (refrigerated)
Arriving >48 hours:  ship on dry ice  
Shipping Requirements: Ship specimens in compliance with governmental regulations.

Billing

CPT Code: 87252 Fees: http://www.dshs.state.tx.us/lab/fees.shtm


Microbiology
Mumps (Culture - Identification)
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Mumps (Culture - Identification)
Test Includes: Immunofluorescence

Reporting

Results Available: 2-14 days Contact #s:512-458-7594

Reference

Method: Immunofluorescence  
Turnaround Time:  2-14 days Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: If transporting on dry ice, send 1-2 mLs. Preferred Specimen:  Cell culture isolate with CPE.
Collection/Preservation: Storage Instructions: If shipment of isolate will be delayed, store at -70° C.
Causes for Rejection: Sample Container: Sterile cryovial.
Sample Test Kit: Availability: Monday - Friday
Diagnostic Information:  Identification based on immunofluorescence test using mumps-specific monoclonal antibody.

Specimen Submission

Required Request Form: G-2V Specimen Handling:
Transport Temperature:  Frozen isolate:  on dry ice Shipping Requirements: Ship specimens in compliance with governmental regulations.

Billing

CPT Code: 87253 Fees: http://www.dshs.state.tx.us/lab/fees.shtm


Microbiology
Mumps (Serological – Enzyme Immunoassay for IgG)
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Mumps (Serological – Enzyme Immunoassay for IgG)
Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method: EIA  
Turnaround Time:  5-7 days Reference Range:  Nonreactive
Limitations: May not detect a recent immunization or infection, or immune status in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.

Specimen Requirements

Specimen Collection: Venipuncture Sample Type:
Volume/Amount Required: 2 mL Serum Preferred Specimen:  Single Serum; Paired Sera
Collection/Preservation: Samples that will be delivered to the laboratory within 8 hours of collection may be transported at room temperature in the original blood collection tube. If the samples are going to be shipped and will be delivered to the laboratory within 48 hours of collection, sera must be separated from the blood and shipped on cold packs, between 2° and 8°C. If the serum samples will not be delivered to the laboratory within 48 hours of collection at these temperatures, then the samples must be frozen at -20°C or lower and shipped on dry ice. Storage Instructions: Separated serum may be held at 2-8°C, or frozen at -20°C.
Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing, specimens received with extended transit time, or received at incorrect temperature. Sample Container: Red top or tiger top tube
Sample Test Kit: Availability: Test is performed once per week
Diagnostic Information:  Acute and convalescent serum specimens are to be collected 14 days apart.  A significant EIA antibody rise is evidence of current or previous infection. Test is performed once per week.

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature: Cold (2-8º C) on ice packs if received within 48 hours from time of collection. Frozen (≤-20°) on dry ice if received more than 48 hours from time of collection. Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code: 86735 Fees: http://www.dshs.state.tx.us/lab/fees.shtm


Microbiology
Mumps (Serological – Immunofluorescence assay for IgM)
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Mumps (Serological – Immunofluorescence assay for IgM)
Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method: EIA  
Turnaround Time:  3-5 days Reference Range:  <1:10 (Nonreactive)
Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.  

Specimen Requirements

Specimen Collection: Venipuncture Sample Type:
Volume/Amount Required: 2 mL Serum Preferred Specimen:  Single Serum; Paired Sera
Collection/Preservation:
Samples that will be delivered to the laboratory within 8 hours of collection may be transported at room temperature in the original blood collection tube. If the samples are going to be shipped and will be delivered to the laboratory within 48 hours of collection, sera must be separated from the blood and shipped on cold packs, between 2° and 8°C. If the serum samples will not be delivered to the laboratory within 48 hours of collection at these temperatures, then the samples must be frozen at -20°C or lower and shipped on dry ice.

* Testing must be completed within one week of the date of collection.
Storage Instructions: Separated serum may be held at 2-8°C, or frozen at -20°C.
Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing Sample Container: Red top or tiger top tube
Sample Test Kit: Availability: Test is performed twice per week.
Diagnostic Information:  The IFA for the serodiagnosis of mumps measures IgM antibodies specific for mumps virus. An IgM titer of 1:10 or greater is suggestive of recent or current mumps virus infection. Test is performed twice per week.

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature:Cold (2-8º C) on ice packs if received within 48 hours from time of collection. Frozen (=-20°) on dry ice if received more than 48 hours from time of collection. Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code: 86735 Fees: http://www.dshs.state.tx.us/lab/fees.shtm


Microbiology
Mycobacteriosis (Culture - Isolation)
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Mycobacteriosis (Culture - Isolation)
Test Includes: Isolation of mycobacteria and identification to species level.

Reporting

Results Available: Positive cultures 14-21 days or earlier; Negative cultures 43 days Contact #s: 512-458-7586 or
512-458-7318 ext 2687

Reference

Method: Primary identification test is mycolic acid analysis by high performance liquid chromatography
Turnaround Time:  Negatives held for six weeks Reference Range:  Negative
Limitations:  Delay in transport of specimen could compromise isolation of organism. Interpretation:

Specimen Requirements

Specimen Collection: Sample Type: Clinical Specimen
Volume/Amount Required: 3 mL to 15 mL Preferred Specimen:  Sputum; Tissue; Exudate; Urine; Stool; Blood; Body Fluids  
Collection/Preservation: No preservative Storage Instructions: Transport specimen as soon as possible.  If transport is delayed over one hour, refrigerate specimen unless blood. 
Causes for Rejection: Specimens received frozen, in formalin, or in culture medium will be rejected.  Swabs are discouraged unless the only specimen available; submit swabs in 5 mL sterile saline.  Gastric specimens must be neutralized prior to transport.  Refrigerated blood specimens will be rejected. Sample Container: Triple contained. Sterile, leak-proof, 50 mL conical tube preferred for primary container. Add up to 10 mL sterile saline to tissue if needed to maintain moisture during transport. If blood, must collect in Yellow-top vacutainer with SPS.
Sample Test Kit: Availability: Tested 5/days: Monday-Friday
Diagnostic Information: Primary diagnostic specimens and isolates go to TDSHS Laboratory in Austin. Direct HPLC (presumptive identification only) performed on positive AFB smear initial diagnostic specimens. Nucleic acid amplification testing for M. tuberculosis complex may be performed on positive AFB smear initial pulmonary specimens if Direct HPLC testing is inconclusive. For more information telephone 512-458-7342

Specimen Submission

Required Request Form: G-2B Specimen Handling:
Transport Temperature:   Ambient Shipping Requirements: Triple-contained and packaged to meet requirements of DOT, USPS, PHS, and IATA for shipping of clinical specimens.

Billing

CPT Code: 87116 Fees:


Microbiology
Mycobacteriosis (Culture - Identification)
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Mycobacteriosis (Culture - Identification)
Test Includes: Identification of isolate by mycolic acid analysis; biochemical testing and/or Accuprobe culture confirmation test performed as adjunct tests if necessary

Reporting

Results Available: 2-25 days Contact #s: 512-458-7586

Reference

Method: Primary identification test is mycolic acid analysis by high performance liquid chromatography.
Turnaround Time:  2-25 days

M. tuberculosis complex reports are telephoned to the submitter on previously undiagnosed patients.

Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Pure Culture
Collection/Preservation: No preservative. Storage Instructions:
Causes for Rejection: Sample Container: Triple-contained
Sample Test Kit: Availability:
Diagnostic Information:  Pure culture isolates submitted to this Laboratory for definitive identification and drug susceptibilities.  Molecular typing performed at regional center upon request. Definitive identification performed on positive isolates by HPLC or a combination of HPLC and biochemical testing or HPLC and genetic probe confirmation.  For more information telephone 512-458-7342

Specimen Submission

Required Request Form: G-2B Specimen Handling:
Transport Temperature:  Ambient Shipping Requirements: Triple-contained and packaged to meet rigorous performance tests as outlined in the DOT, USPS, PHS, and IATA regulations for shipping of infectious substances.

Billing

CPT Code: 87118 Fees: http://www.dshs.state.tx.us/lab/fees.shtm


Microbiology
Mycobacteriosis (Culture – Susceptibility, Primary Drug Panel)
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Mycobacteriosis (Culture – Susceptibility, Primary Drug Panel)
Test Includes:  Drug Susceptibility Testing of Mycobacterium by Indirect Drug Susceptibility Test

Reporting

Results Available: 10 days Contact #s: 512-458-7586

Reference

Method: BACTEC indirect drug susceptibility test system for M. tuberculosis complex. 
Agar proportion indirect drug susceptibility test system for M. kansasii
Turnaround Time: 10-28 days (28 days for confirmation of resistance). Reports of drug resistance are telephoned to the submitter. Reference Range:  Susceptible
Limitations: Test can only be performed on pure cultures of mycobacteria. Interpretation: Report states isolate is either susceptible or resistant to a specific drug at critical concentrations recommended by NCCLS.

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Pure Culture or Isolate
Collection/Preservation: No preservative. Storage Instructions:
Causes for Rejection: Sample Container: Triple-contained.
Sample Test Kit: Availability: Test is set up 5 days/week: Monday – Friday.  Test is run 7 days/week.
Diagnostic Information:  Pure culture isolates of M. tuberculosis complex or M. kansasii submitted to this Laboratory for drug susceptibilities. Isolates submitted for drug susceptibilities will be identified by this Laboratory. No fee will be charged for the identification test unless the AFB ID test is requested on form G-2B. Primary Panel for M. tuberculosis complex consists of Isoniazid, Ethambutol, or Rifampin testing by BACTEC susceptibility testing. Resistance to any of these drugs is confirmed by agar proportion method. If resistance to any of these drugs is detected, the Secondary Susceptibility Panel is performed. Primary Panel for M. kansasii consists of Rifampin testing by agar proportion method. For drug susceptibility testing of other Mycobacterium species, please telephone 512-458-7342.  Pure cultures of other Mycobacterium species may be forwarded by TDSHS laboratory to other reference laboratories for susceptibility testing.  A handling fee may be charged.  Testing at other reference laboratories is subject to fees charged by those laboratories.

Specimen Submission

Required Request Form: G-2B Specimen Handling:
Transport Temperature:  Ambient Shipping Requirements: Triple-contained and packaged to meet rigorous performance tests as outlined in the DOT, USPS, PHS, and IATA regulations for shipping of infectious substances.

Billing

CPT Code: 87188 Fees: http://www.dshs.state.tx.us/lab/fees.shtm


Microbiology
Mycobacteriosis (Culture – Susceptibility, Secondary Drug Panel)
< Return to Table of Contents
Mycobacteriosis (Culture – Susceptibility, Secondary Drug Panel)
Test Includes: Drug Susceptibility Testing of Mycobacterium by Indirect Drug Susceptibility Test

Reporting

Results Available: 10-28 days Contact #s: 512-458-7586

Reference

Method: BACTEC indirect drug susceptibility test system for M. tuberculosis complex.
Agar proportion indirect drug susceptibility test system for confirmation of drug resistance.
Turnaround Time:  10-28 days. Reports of drug resistance are telephoned to the submitter. Reference Range:  Susceptible
Limitations:  Testing can only be performed on pure cultures of Mycobacterium tuberculosis complex. Interpretation: Report states isolate is either susceptible or resistant to a specific drug at critical concentrations recommended by NCCLS. 

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Pure Culture
Collection/Preservation: No preservative. Storage Instructions:
Causes for Rejection: Sample Container: Triple-contained
Sample Test Kit: Availability: Test is set up 5 days/week:  Monday- Friday.  Test is run 7 days/week.
Diagnostic Information:  Pure culture isolates of M. tuberculosis complex submitted or isolated at this Laboratory for drug susceptibilities. Isolates submitted for drug susceptibilities will be identified by this Laboratory. No fee will be charged for the identification test unless the AFB ID test is requested on form G-2B. Secondary Panel for M. tuberculosis complex consists of Streptomycin, Ethionamide, Kanamycin, Pyrazinamide, and Ofloxacin by BACTEC susceptibility testing. Resistance to any of these drugs is confirmed by the agar proportion method. Capreomycin is available by agar proportion method only. For drug susceptibility testing of other Mycobacterium species, please telephone 512-458-7342.  Pure cultures of other Mycobacterium species may be forwarded by TDSHS laboratory to other reference laboratories for susceptibility testing.  A handling fee may be charged.  Testing at other reference laboratories is subject to fees charge by those laboratories.

Specimen Submission

Required Request Form: G-2B Specimen Handling:
Transport Temperature:  Ambient Shipping Requirements: Triple-contained and packaged to meet rigorous performance tests as outlined in the DOT, USPS, PHS, and IATA regulations for shipping of infectious substances.

Billing

CPT Code: 87190 Fees: http://www.dshs.state.tx.us/lab/fees.shtm


Microbiology
Mycobacteriosis (Culture – Susceptibility, Pyrazinamide Only)
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Mycobacteriosis (Culture – Susceptibility, Pyrazinamide Only)
Test Includes: Drug susceptibility testing of Mycobacterium tuberculosis complex to pyrazinamide.

Reporting

Results Available: 7-28 days Contact #s: 512-458-7586

Reference

Method: BACTEC indirect drug susceptibility test system  
Turnaround Time:  7-28 days
Reports of drug resistance are telephoned to the submitter.
Reference Range:  By report
Limitations: Test can only be performed on pure cultures of M. tuberculosis complex Interpretation: Report states that the isolate is either susceptible or resistant to pyrazinamide at critical concentration recommended by NCCLS.

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Pure Culture
Collection/Preservation: No preservative. Storage Instructions:
Causes for Rejection: Sample Container: Triple-contained.
Sample Test Kit: Availability: Test is set up 5 days/week:  Monday-Friday.  Test is run 7 days/week.
Diagnostic Information:  Pure culture isolates of M. tuberculosis complex submitted or isolated at this Laboratory for drug susceptibilities. Isolates submitted for drug susceptibilities will be identified by this Laboratory. No fee will be charged for the identification test unless the AFB ID test is requested on form G-2B. Pyrazinamide testing is performed by BACTEC susceptibility testing.

Specimen Submission

Required Request Form: G-2B Specimen Handling:
Transport Temperature: Ambient  Shipping Requirements: Triple-contained and packaged to meet rigorous performance tests as outlined in the DOT, USPS, PHS, and IATA regulations for shipping of infectious substances.

Billing

CPT Code: 87190 Fees: http://www.dshs.state.tx.us/lab/fees.shtm


Microbiology
Mycobacteriosis (Culture – Isolation - Direct Nucleic Acid Amplification Test (NAAT) for Mycobacterium tuberculosis complex)
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Mycobacteriosis (Culture – Isolation - Direct Nucleic Acid Amplification Test (NAAT) for Mycobacterium tuberculosis complex)
Test Includes: Nucleic Acid Amplification Testing of Clinical Specimens for Mycobacterium tuberculosis complex

Reporting

Results Available: 48 hours Contact #s: 512-776-7342 or 512-776-7580

Reference

Method: Cepheid GeneXpert PCR test amplifies and detects DNA of M. tuberculosis complex
Turnaround Time: 48 hours from specimen receipt in this laboratory. Positive reports are communicated to the specimen submitter on day of testing. Reference Range:   Normal value = Negative
Limitations: Specimen must be sputum, induced or expectorated. AFB Smear and Culture always performed concurrently in this laboratory. A negative test does not mean the patient does not have M. tuberculosis disease.Test results must be confirmed by culture of specimen for the presence of mycobacteria. If a processed sputum sediment is submitted for NAAT only, the AFB culture must be in progress at the submitting laboratory site. Interpretation: A Positive result means that the DNA of the M. tuberculosis complex is detected. A Negative result means that the DNA of the M. tuberculosis complex was not detected. A Negative result does not rule out the possibility of the presence of viable M. tuberculosis complex in the specimen.

Specimen Requirements

Specimen Collection: Follow the Guidelines for Specimen Collection and Submission: Mycobacteriology (AFB) Collection, Transport, and Storage. Sample Type: Clinical
Volume/Amount Required: 3 mL to 15 mL Preferred Specimen:  Sputum or processed sputum sediment. For all other specimen types, please contact 512-776-7342 or 512-776-7580.
Collection/Preservation: No preservative Storage Instructions: Transport specimen as soon as possible. Diagnostic specimens should be shipped on cold pack overnight.
Causes for Rejection: Specimen leaked in transit. No identifier on specimen container. No test requested on G-MYCO requisition form.  Sample Container:Triple contained. Sterile, leak-proof, 50 mL conical tube preferred for primary container.
Sample Test Kit: Availability: Monday through Friday
Diagnostic Information: There should be a high clinical suspicion of tuberculosis when ordering this test. NAA testing should be requested if the test results will make a difference to the patient’s treatment or if the test results will make a difference to the contact investigation. Patients should not have a laboratory diagnosis of tuberculosis disease already.  

Specimen Submission

Required Request Form: G-MYCO
  • For sputum specimens, check test “AFB Smear, Culture and Direct NAAT (Respiratory Diagnostic Specimens Only)”
  • For processed sputum sediments, check test “Direct NAAT for M. tuberculosis”
Specimen Handling: Follow the Guidelines for Specimen Collection and Submission: Mycobacteriology (AFB) Collection, Transport, and Storage.
Transport Temperature: Cold shipment (not frozen) recommended. Shipping Requirements: Triple-contained and packaged to meet requirements of DOT, USPS, PHS, and IATA for shipping of clinical specimens. Please check with your courier of choice.

Billing

CPT Code: 87556 Fees: http://www.dshs.state.tx.us/lab/fees.shtm


Microbiology
Mycobacteriosis (Microscopic – Truant’s Stain)
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Mycobacteriosis (Microscopic – Truant’s Stain)
Test Includes: Microscopic Examination of Clinical Specimen for Acid Fast Bacilli (AFB)

Reporting

Results Available: 1 day. Positive reports are telephoned to the submitter within two hours of availability. Contact #s: 512-458-7586

Reference

Method: Truant’s Acid Fast Stain  
Turnaround Time:  24 hours Reference Range:  Negative
Limitations:  A negative microscopic examination does not mean that the patient’s specimen cannot be culture positive for AFB.  This test is less sensitive than culture.  The stain utilizes a fluorescent dye that can be taken up by the mycobacterium to different degrees depending on the species or if the patient is on antibiotic treatment.  The quality of the specimen has major impact on the results of the microscopic examination. Interpretation:  The report states whether the test is Negative if No AFB are Seen or Positive if AFB are Seen on microscopic examination.  If Positive, the report provides a quantitation to indicate how many AFB were seen in a high power field under the microscope.  Occasionally, an examination will result in the observation of only one or two AFB in the entire test.  This number of AFB (one or two) is reported but is not considered a positive result by CDC or the American Thoracic Society.

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: 3 mL to 15 mL
Preferred Specimen:  Sputum; Tissue; Exudate; Stool; Blood; Body Fluids; Urine
Collection/Preservation: No preservative Storage Instructions: Transport specimen as soon as possible.  If transport is delayed over one hour, refrigerate specimen, unless blood.  This is important if the specimen is also to be cultured.
Causes for Rejection: Specimens received frozen, in formalin, or in culture medium will be rejected.  Swabs are discouraged unless the only specimen available; submit swabs in 5 mL sterile saline.  If swabs are submitted in transport medium, the test cannot be performed. Sample Container: Triple contained. Sterile, leak-proof, 50 mL conical tube preferred for primary container. Add up to 10 mL of sterile saline to tissue if needed to maintain moisture during transport. If blood, must collect in Yellow-top vacutainer with SPS.
Sample Test Kit: Availability: Tested 5 days/week:  Monday-Friday
Diagnostic Information:  Primary diagnostic specimens and isolates go to TDSHS Lab in Austin.

Specimen Submission

Required Request Form: G-2B Specimen Handling:
Transport Temperature:   Ambient Shipping Requirements: Triple-contained and packaged to meet requirements of DOT, USPS, PHS, and IATA for shipping of clinical specimens.

Billing

CPT Code: 87206 Fees: http://www.dshs.state.tx.us/lab/fees.shtm


Microbiology
Mycetoma (Culture – Isolation)
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Mycetoma (Culture – Isolation)
Test Includes: Isolation of fungus and identification by morphological tests.

Reporting

Results Available: 21-28 days Contact #: 512-458-7455 for prior approval
Technical questions:  512-458-7586

Reference

Method: Slide culture  
Turnaround Time:  21-28 days Reference Range:  Negative
Limitations: Delay in transport of specimen could compromise isolation of organism. Interpretation:

Specimen Requirements

Specimen Collection: Sample Type: Clinical Specimen
Volume/Amount Required: Preferred Specimen:  Tissue; Exudate
Collection/Preservation: No preservative Storage Instructions: Transport specimen as soon as possible.  If transport is delayed over one hour, refrigerate specimen. 
Causes for Rejection: Specimens received frozen, in formalin, or in culture medium will be rejected.  Swabs are discouraged unless the only specimen available; submit swabs in 5 mL sterile saline. Sample Container: Triple-contained.  Sterile, leak-proof, 50 mL conical tube preferred for primary container.  Add no more than 10 mL sterile saline to tissue if needed to maintain moisture during transport.
Sample Test Kit: Availability:  Testing available upon approval of Dr. Penfield.
Diagnostic Information:  Approval for this testing must be obtained prior to shipping by telephoning Dr. Susan Penfield at 512-458-7455.

Specimen Submission

Required Request Form: G-2B Specimen Handling:
Transport Temperature:  Ambient acceptable but 2-8º C preferred for non-sterile specimens. Shipping Requirements: Triple-contained and packaged to meet requirements of DOT, USPS, PHS, and IATA for shipping of clinical specimens.

Billing

CPT Code: 87102 Fees: http://www.dshs.state.tx.us/lab/fees.shtm


Microbiology
Mycetoma (Culture – Identification)
< Return to Table of Contents
Mycetoma (Culture – Identification)
Test Includes: Identification of fungus by morphological tests

Reporting

Results Available: 21-28 days Contact #s: 512-458-7586

Reference

Method: Slide Culture  
Turnaround Time:  21-28 days Reference Range:  By report
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: Sample Type:
Volume/Amount Required: Preferred Specimen:  Pure Culture
Collection/Preservation: No preservative. Storage Instructions:
Causes for Rejection: Culture infested with mites. Sample Container: Triple-contained.
Sample Test Kit: Availability:  Tested 5 days/week:  Monday-Friday
Diagnostic Information:    Fungal isolates (pure culture) submitted to this Laboratory for definitive identification. Drug susceptibility testing on these organisms not available at TDSHS laboratory.

Specimen Submission

Required Request Form: G-2B Specimen Handling:
Transport Temperature:  Room temperature Shipping Requirements: Triple-contained and packaged to meet rigorous performance tests as outlined in the DOT, USPS, PHS, and IATA regulations for shipping of infectious substances.

Billing

CPT Code: 87102 Fees: http://www.dshs.state.tx.us/lab/fees.shtm


Microbiology
Mycoplasmosis (Serological – Forwarded by TDSHS to CDC for testing.)
< Return to Table of Contents
Mycoplasmosis (Serological – Forwarded by TDSHS to CDC for testing.)
Test Includes:

Reporting

Results Available: Contact #s:

Reference

Method:  
Turnaround Time:  3 weeks Reference Range:  <1:8 (Nonreactive)
Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.

Specimen Requirements

Specimen Collection: Venipuncture Sample Type:
Volume/Amount Required: 10 mL whole blood Preferred Specimen:  Single serum; Paired sera
Collection/Preservation: Red top or tiger top tube Storage Instructions: Do not freeze or refrigerate whole blood. Separated serum may be held at 2-8°C
Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing Sample Container: Red top or tiger top tube
Sample Test Kit: Availability:
Diagnostic Information:  Previous notification is requested (458-7760). A detailed patient history is required.  Single titers of >1:64 are indicative of recent infection. Titers of 1:8 to 1:32 may be indicative of either past or present infection. A four-fold or greater increase in titer between acute and convalescent specimens confirms the diagnosis.

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature:  Ambient temperature for specimens on the blood clot, separated serum at 2-8°C (refrigerated) or -20°C (frozen). Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code: 86738 Fees: http://www.dshs.state.tx.us/lab/fees.shtm



Last updated February 20, 2014