Fluorescent Antibody Test for Rabies
Touch impressions of brain material are prepared on microscope slides using three parts of the brain: cerebellum, hippocampus, and brain stem. The slides are fixed in acetone for at least one hour. Next, the slides are stained using a fluorescein labeled antibody specific for rabies virus, which is diluted in a normal rabbit brain suspension, and incubated with the antibody for 30 minutes. They are then washed in PBS two times, for five minutes each, dipped in distilled water, and air-dried. The slides are mounted with buffered glycerol, pH 9.0, and examined using an epifluorescent microscope. Positive and negative control slides are included with each run.