EHEC


Enterhaemorrhagic Esherichia Coli (EHEC), Shiga-like Toxin assay

Laboratory Fee Schedule

Procedure #:  MAA0047A

CPT:    87427


Enterhaemorrhagic Esherichia Coli (EHEC), Shiga-like Toxin assay

Synonym(s):

Shiga-like toxin, ST-1 ST-2, STEC, EHEC, E. coli toxin

Requisition Form

G-2B

Test Description

Escherichia coli shiga toxin assay

Pre-Approval Needed

N/A

Supplemental Information Required

N/A

Supplemental Form(s)

N/A

Performed on Specimens from (sources)

Human

Sample/Specimen Type for Testing

Pure culture E. coli; Clinical stool

Minimum Volume/Size Required

Pure culture

  • At least one viable colony

Clinical sample

  • 500 µl


Storage/Preservation Prior to Shipping

Pure Culture: 

  • Refrigerated

Broth/liquid transport

  • Refrigerated: Received < 7 days
  • Frozen @-70C: Received > 7 days
 Transport Medium
  • Media supporting growth of isolate
  • Clinical sample in GN broth or transport media

Specimen Labeling

  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match submission form.

Shipping and Specimen Handling Requirements

  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
  • Ship specimens overnight Monday-Thursday, to avoid weekend deliveries.

Method

Enzyme Immunoassay

Turn-around Time

3-21 days

Interferences/Limitations

  • Material must be viable.  
  • Improper storage or shipping temperatures limit organism recovery. 

Common Causes for Rejection

  • Insufficient quantity
  • Unacceptable specimen type or source
  • Improper shipping conditions
  • Expired media or collection container
  • Discrepancies between specimen label and submission form
  • Incomplete or missing submission form.

Additional Information

N/A

Last updated March 3, 2020