AFBhplc

Acid Fast Bacilli Isolate Identification by High 

Performance Liquid Chromatography (HPLC)   

Laboratory Fee Schedule

Procedure: MAC0052A

CPT: 87143


Acid Fast Bacilli Isolate Identification by High Performance Liquid Chromatography (HPLC)
Synonym(s): HPLC Identification of clinical isolates
Requisition Form G-MYCO
Test Description Mycolic acid analysis identification of mycobacterial isolates cultured from clinical specimens in DSHS Laboratory
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A  
Performed on Specimens from (sources) Pure isolate from human sources
Sample/Specimen Type for Testing Mycobacterium species recovered by DSHS laboratory in liquid or on solid culture medium
Minimum Volume/Size Required

•   Liquid culture- 2.0 mL

•   Solid culture- Single mature colony

Storage/Preservation Prior to Shipping N/A
Transport Medium N/A
Specimen Labeling

•   At least 2 patient specific identifiers:  First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. Medical records number).

•   Specimen identification must match specimen submission form.

Shipping and Specimen Handling Requirements

•   N/A

Method High performance liquid chromatography
Turn-around Time 2 days
Interferences/Limitations

•   HPLC may only detect the predominant organism in a mixed culture.

•   Liquid cultures must be instrument positive in order to analyze by HPLC.

•   Additional testing methods may need to be used to provide definitive identification

Common Causes for Rejection N/A
Additional Information

•   This test is used in-house to identify Mycobacterium species cultured from submitted clinical specimens.

Last updated January 9, 2019