AFBspecimen

Acid Fast Bacilli (AFB) Specimen Concentration

Laboratory Fee Schedule

Procedure: MAG0066A

CPT: 87015


Acid Fast Bacilli (AFB) Specimen Concentration
Synonym(s): Mycobacteria specimen concentration, specimen decontamination, specimen digestion
Requisition Form G-MYCO
Test Description Processing of non-sterile specimens for the growth and detection of mycobacteria
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Sputum; Exudate; Stool; Badly contaminated tissues/body fluids
Sample/Specimen Type for Testing Clinical specimen
Minimum Volume/Size Required 3 mL to 15 mL
Storage/Preservation Prior to Shipping Transport specimen as soon as possible on cold pack. If transport is delayed over one hour, refrigerate specimen.
Transport Medium Do not use transport medium. A sterile, leak-proof, 50 mL conical tube preferred for primary container. Add up to 10 mL sterile saline to tissue if needed to maintain moisture during transport.
Specimen Labeling

•   At least 2 patient specific identifiers: First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. Medical records number).

•   Specimen identification must match specimen submission form.

Shipping and Specimen Handling Requirements

•   Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49

•    Category B shipping applies

•    Ship specimens overnight and preferably on cold pack.

Method N-Acetyl-L-cysteine (NALC)- sodium hydroxide (NaOH) digestion-decontamination
Turn-around Time N/A
Interferences/Limitations Delay in transport of specimen could compromise isolation of organism.
Common Causes for Rejection Specimen leaked in transit. No identifier on specimen container. No test requested on G-MYCO requisition form. Specimens received frozen, in formalin, or in culture medium will be rejected. Swabs are discouraged unless the only specimen available; submit swabs in 5 mL sterile saline. Gastric specimens must be neutralized prior to transport.
Additional Information Mycobacteria are recovered optimally from clinical specimens when methods both to release them from body fluids and cells (digestion) and remove/reduce competing organisms (decontamination) are used.

Last updated January 9, 2019