Arbovirus IgM EIA, St. Louis Encephalitis and West Nile

Laboratory Fee Schedule

Procedure: MAM0460A, MZZ0082A

CPT: 86653, 86788

Arbovirus IgM EIA, St. Louis Encephalitis and West Nile
Synonym(s): Capture Enzyme Immunoassay for the Detection of IgM Antibodies to West Nile and St. Louis Encephalitis Viruses
Requisition Form G-2A
Test Description Capture Enzyme Immunoassay (EIA) to detect the presence of specific IgM antibodies
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Human
Sample/Specimen Type for Testing Serum or Cerebral Spinal Fluid (CSF)
Minimum Volume/Size Required 1.0 mL
Storage/Preservation Prior to Shipping Specimen should be frozen at -20°C or colder or may be stored between 2°C to 8°C if it will be tested within two days.
Transport Medium N/A
Specimen Labeling At least 2 patient specific identifiers: First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. Medical Record number); must match information on submission form (G-2A)
Shipping and Specimen Handling Requirements

•  Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.

•  Handle as infectious agent using universal precautions.

•  Triple contain in accordance with federal shipping regulations for infectious agents. Additional forms & resources

Method Capture EIA
Turn-around Time 8 days
Interferences/Limitations Cross-reactions can occur between flaviviruses. Specific IgG can compete with IgM and may result in a false negative. Rheumatoid factor in presence of specific IgG may result in a false positive.
Common Causes for Rejection Insufficient quantity, unacceptable specimen type or source, improper shipping conditions, expired media or collection container, discrepancies between specimen label and submission form, and/or incomplete or missing submission form.
Additional Information Convalescent serum specimen should be tested if an early acute CSF or serum is negative. For most, IgM is detectable 8 days post-onset of symptoms and can persist for at least 45 days and up to 90 days.

Last updated January 9, 2019