Acid Fast Bacilli (AFB) Culture

Laboratory Fee Schedule

Procedure: MZZ0062A

CPT: 87116

Acid Fast Bacilli (AFB) Culture
Synonym(s): Mycobacterial culture, isolation
Requisition Form G-MYCO
Test Description Isolation of mycobacteria from culture 
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Sputum; Tissue; Exudate; Stool; Body Fluids
Sample/Specimen Type for Testing Clinical specimen from human
Minimum Volume/Size Required 3 mL to 15 mL
Storage/Preservation Prior to Shipping Transport specimen as soon as possible on cold packs. If transport is delayed over one hour, refrigerate specimen.
Transport Medium Do not use transport medium.  A sterile, leak-proof, 50 mL conical tube preferred for primary container.  Add up to 10 mL sterile saline to tissue, if needed to maintain moisture during transport.
Specimen Labeling

•   At least 2 patient specific identifiers: First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. Medical records number).

•   Specimen identification must match specimen submission form.

Shipping and Specimen Handling Requirements

Please ship specimens overnight and preferably on cold pack.

•   Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49

•   Category B shipping applies

Method Culture is performed on solid Middlebrook medium and in the liquid BACTEC MGIT culture medium
Turn-around Time All cultures are monitored for 42 days, or until positive.
Interferences/Limitations Delay in transport of specimen could compromise isolation of organism.
Common Causes for Rejection Specimen leaked in transit. No identifiers on specimen container. No test requested on G-MYCO requisition form. Specimens received frozen, in formalin, or in culture medium will be rejected. Swabs are discouraged unless the only specimen available; submit swabs in 5 mL sterile saline. Gastric specimens must be neutralized prior to transport.
Additional Information  

Last updated January 9, 2019