Brucella IgG/IgM Agglutination

Laboratory Fee Schedule

Procedure:  MZZ0135A

CPT: 86622

Brucella IgG/IgM Agglutination
Synonym(s): DS Microtiter Procedure for Brucella Agglutination Test
Requisition Form G-2A
Test Description The detection and quantitation of B. abortus IgG and IgM antibodies by the agglutination of safranin-O stained whole cells
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Any
Sample/Specimen Type for Testing Blood, serum or plasma
Minimum Volume/Size Required 1.0 mL
Storage/Preservation Prior to Shipping

Allow blood to clot at room temperature before centrifuging.   Serum can be used if particulates are removed via filtration or by using lipid clearing reagents and centrifugation. Plasma can be stored at:

•   2°C  to 8°C  if tested within two days or

•   Frozen at ≤ -15°C or colder if not tested within 2 days

Transport Medium N/A
Specimen Labeling

•   At least 2 patient specific identifiers: First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. Medical Record number) 

•   Must match information on submission form (G-2A).

Shipping and Specimen Handling Requirements

•   Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.

•   Handle as infectious agent using universal precautions.

•   Triple contain in accordance with federal shipping regulations for infectious agents.
Additional forms & resources

Method Antibody agglutination test
Turn-around Time 7 days
Interferences/Limitations Hemolytic, hyperlipemic and bacterial contamination can produce erroneous results in serologic tests.
Common Causes for Rejection Insufficient quantity, unacceptable specimen type or source, improper shipping conditions, expired media or collection container, discrepancies between specimen label and submission form, and/or incomplete or missing submission form.
Additional Information This test can provide presumptive evidence of Brucellosis.  Results should be considered in combination with other clinical, laboratory and epidemiologic findings.

Last updated November 28, 2018