Laboratory Fee Schedule

Procedure #: BZZ0015A

CPT: 82465

Synonym(s): Total cholesterol
Requisition Form G-1B, G-THSTEPS
Test Description The quantitation of cholesterol in human serum is used to determine the risk of coronary heart disease.
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Human
Sample/Specimen Type for Testing

•   Preferred: Serum from a red top tube transferred to a transport tube

•   Acceptable: Serum from a centrifuged gold top serum separator tube.

Minimum Volume/Size Required 1mL serum
Storage/Preservation Prior to Shipping

•   Collect blood in red top or gold top tube.

•   Gently invert the tube with the blood specimen five times immediately after collection.

•   Allow to clot 30 minutes from collection. 
•   Centrifuge gold top tube to separate serum from red blood cells.
•   For red top tube:  Immediately transfer serum into a transport tube. 
•   For gold top tube:  Immediately transfer serum into a transport tube if serum will be frozen.*
•   Store serum cold if testing will occur within 7 days of collection.
•   Store serum frozen if testing will occur after 7 days of collection.

NOTE: * Gold top serum separator tube cannot be frozen. Serum must be transferred to a transport tube prior to freezing.  

Transport Medium N/A
Specimen Labeling

•   Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number).

•   Identifiers on specimen must exactly match submission form.

Shipping and Specimen Handling Requirements

•   Ship all specimens overnight with enough cold packs or dry ice to maintain specimens at proper temperature until arrival at DSHS Laboratory.
•   Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
•   Handle as infectious agent using universal precautions.
•   Ship triple-contained in accordance with federal shipping regulations for infectious agents.
•   Ship Monday-Thursday; avoid Friday and weekend deliveries
•   Ship cold specimens overnight on ice packs if arriving at laboratory within 7 days from collection. 
•   Ship frozen specimens overnight on dry ice or on ice packs.
•   Specimens must be received cold/frozen by the laboratory.   
•   Consider freezing serum transport tubes prior to shipping during high temperature months.

Method Quantitative enzymatic
Turn-around Time 4-5 working days after receipt of specimen
Interferences/Limitations Lipemia, turbidity, icterus, hemolysis, metamizole, N-acetyl cysteine.
Common Causes for Rejection

•    Two patient-specific identifiers not met
•    Specimen received at ambient temperature due to inadequate/no ice packs.
•    Specimen unsuitable for testing
•    Specimen was received at improper temperature
•    No specimen included with submission form. 
•    Submission form not included with specimen. 
•    Specimen tube broken/leaked in transit. 
•    Demographic information is missing or incorrect. 
•    Specimen identification is missing or incorrect.
•    Whole blood was received. 
•    Specimen is collected in an expired blood collection tube. 
•    No test specified.
•    Red top tubes have not had serum poured over to a transport tube.
•    Frozen gold top tubes.

Additional Information 

Reference Range:

•   Children/adolescents (<20 years): <170 mg/dL

•   Young adults (20-24 years): <190 mg/dL

•   Adults: <200 mg/dL


•   Telephone: 512-776-6236 or 512-776-2628

•   Fax: 512-776-7221

•   Email:

Last updated May 9, 2022