DirectHPLC

Direct Detection by High Performance Liquid Chromatography (HPLC)  

Laboratory Fee Schedule

Procedure: MAF0064A

CPT: 87143


Direct Detection by High Performance Liquid Chromatography (HPLC)
Synonym(s): Direct HPLC
Requisition Form G-MYCO
Test Description Mycolic acid analysis identification of AFB in smear-positive processed specimens
Pre-Approval Needed Yes; call 512-776-7342 for pre-approval
Supplemental Information Required N/A
Supplemental Form(s) N/A  
Performed on Specimens from (sources) Clinical specimens from humans
Sample/Specimen Type for Testing Specimens must be concentrated and smear positive
Minimum Volume/Size Required Processed sediment volume >/= 1 mL
Storage/Preservation Prior to Shipping N/A
Transport Medium N/A
Specimen Labeling

•   At least 2 patient specific identifiers:  First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. Medical records number).

•   Specimen identification must match specimen submission form.

Shipping and Specimen Handling Requirements

•   Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49

•   Category B shipping applies

Method High performance liquid chromatography
Turn-around Time 1 day
Interferences/Limitations

•   Direct HPLC may only detect the predominant organism in a mixed population.

•   Direct HPLC is not highly sensitive and will not be conclusive on specimens that have negative or weakly AFB positive smears.

•   Direct HPLC cannot determine the viability of organisms detected.

•   Direct HPLC results that are negative for M. tuberculosis complex do not conclusively rule out the presence of viable M. tuberculosis complex in the specimen.

Causes for Rejection Volume insufficient for testing
Additional Information

•   Processed specimen sediments may be accepted for testing following pre-approval but AFB smear and culture must be performed concurrently in the submitting laboratory.

Last updated November 5, 2018