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Synonym(s):
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Heterotrophic
plate count (HPC); SimPlate
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Requisition Form
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G-19
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Test Description
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Enumerates
the amount of heterotrophic organisms found in 1 mL of sample using a most probable
number (MPN) statistical value
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Pre-Approval Needed
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N/A
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Supplemental Information Required
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N/A
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Supplemental Form(s)
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N/A
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Performed on Specimens from (sources)
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Food |
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Sample/Specimen Type for Testing
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Surface
water samples
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Minimum Volume/Size Required
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100
mL
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Storage/Preservation Prior to Shipping
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- Specimen
should be maintained at 1.0° to 8.0°C.
- Do
not freeze.
- Sample
must be collected in sterile container and have sufficient head space (~1 inch) for proper
mixing.
- A
temperature control (TC) must be included, using the same container type,
with every set of samples shipped; it should be approximately the same
volume.
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Transport Medium
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N/A
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Specimen Labeling
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Sample
Identification must match submission form.
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Shipping and Specimen Handling Requirements
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- Ship samples on Ice and/or Ice Packs.
- Samples must be delivered directly to the laboratory
within 8 hours of collection.
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Method
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IDEXX
SimPlate™ HPC Test Method for
Heterotrophs
in Water, November 2000.
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Turn-around Time
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3
Days
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Interferences/Limitations
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- This
method detects heterotrophic organisms at a level between 2 MPN/mL and 7,380
MPN/mL.
- Sample
must be tested within 8 hours of collection.
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| Common Causes for Rejection
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-
Insufficient
specimen
- improperly stored
- failure to meet shipping guidelines
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Additional Information
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N/A
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