Rickettsial Panel (RMSF, Typhus), IgG

Rickettsial Panel (RMSF, Typhus), IgG 

Laboratory Fee Schedule 

Procedure #:  MCR0317A 

CPT: 87040, 87070, 87077

Rickettsial Panel (RMSF, Typhus), IgG
 Synonym(s):  Rickettsia Rickettsii and Rickettsia Typhi IgG Antibody Detection by Indirect Immunofluorescence Assay (IFA)
 Requisition Form G-2A
 Test Description Standard IFA using a two stage sandwich immunofluorescence technique for the detection of IgG antibodies to Rocky Mountain Spotted Fever and/or Typhus Fever antigens.
 Pre-Approval Needed N/A
 Supplemental Information Required N/A
 Supplemental Form(s)
 Performed on Specimens from (sources) Human
 Sample/Specimen Type for Testing Serum is preferred, plasma is accepted
 Minimum Volume/Size Required 1.0 mL
 Storage/Preservation Prior to Shipping Specimens may be stored at 2°C to 8°C if testing will occur within 2 days; otherwise store frozen at -15°C or colder 
 Transport Medium N/A
 Specimen Labeling At least 2 patient specific identifiers: First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. Medical Record number); must match information on submission form (G-2A).
 Shipping and Specimen Handling Requirements
  •  Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
  • Handle as infectious agent using universal precautions. 
  • Triple contained in accordance with federal shipping regulations for infectious agents.  
    Additional forms & resources
 Method Immunofluorescence Assay for the detection of antibodies
 Turn-around Time 5 working days
 Interferences/Limitations Gross hemolysis, lipemia , bacterial contamination
 Common Causes for Rejection Insufficient quantity, unacceptable specimen type or source, improper shipping conditions, expired media or collection container, discrepancies between specimen label and submission form, and/or incomplete or missing submission form
 Additional Information In order to confirm an infection was recent, a convalescent phase sample should be submitted.  The most convincing evidence of recent rickettsial disease is a fourfold rise in antibody titer between an acute phase serum and a convalescent phase serum. 

Last updated April 22, 2022