Synonym(s):
|
Whooping
cough
|
Requisition Form
|
G-2B
|
Test Description
|
Real-time
PCR for B. pertussis, B. parapertussis,
B. holmesii
|
Pre-Approval Needed
|
N/A
|
Supplemental Information Required
|
N/A
|
Supplemental Form(s)
|
N/A
|
Performed on Specimens from (sources)
|
Humans
|
Sample/Specimen Type for Testing
|
- Nasopharyngeal
swabs
- Nasopharyngeal
washes in 0.85% saline
- Bacterial
isolates
|
Minimum Volume/Size Required
|
- Nasopharyngeal
swabs: N/A
- Nasopharyngeal
wash: 200µL
|
Storage/Preservation Prior to Shipping
|
- Must
be a rayon or dacron swab with metal or wooden handle with no transport
media.
- Must
be maintained between 2-8°C. Do not freeze.
- Must
be received at laboratory within seven (7) days.
|
Transport Medium
|
|
Specimen Labeling
|
-
Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form.
|
Shipping and Specimen Handling Requirements
|
- Ship according to Dangerous Goods Regulations,
IATA, and/or CFR 49.
- Must
be maintained between 2-8°C. Do not freeze.
- Must be
received on ice packs.
|
Method
|
|
Turn-around Time
|
48 business
hours from receipt
|
Interferences/Limitations
|
Heme
from hemoglobin interferes with PCR in this assay.
|
Common Causes for Rejection
|
-
Insufficient
specimen,
- Bloody swab,
- Stored in transport media,
- Improperly stored,
- Failure
to meet shipping/transportation
|
Additional Information
|
N/A
|
|
|