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Synonym(s):
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Whooping
cough
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Requisition Form
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G-2B
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Test Description
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Real-time
PCR for B. pertussis, B. parapertussis,
B. holmesii
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Pre-Approval Needed
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N/A
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Supplemental Information Required
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N/A
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Supplemental Form(s)
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N/A
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Performed on Specimens from (sources)
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Humans
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Sample/Specimen Type for Testing
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- Nasopharyngeal
swabs
- Nasopharyngeal
washes in 0.85% saline
- Bacterial
isolates
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Minimum Volume/Size Required
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- Nasopharyngeal
swabs: N/A
- Nasopharyngeal
wash: 200µL
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Storage/Preservation Prior to Shipping
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- Must
be a rayon or dacron swab with metal or wooden handle with no transport
media.
- Must
be maintained between 2-8°C. Do not freeze.
- Must
be received at laboratory within seven (7) days.
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Transport Medium
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Specimen Labeling
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-
Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form.
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Shipping and Specimen Handling Requirements
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- Ship according to Dangerous Goods Regulations,
IATA, and/or CFR 49.
- Must
be maintained between 2-8°C. Do not freeze.
- Must be
received on ice packs.
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Method
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Turn-around Time
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48 business
hours from receipt
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Interferences/Limitations
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Heme
from hemoglobin interferes with PCR in this assay.
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| Common Causes for Rejection
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-
Insufficient
specimen,
- Bloody swab,
- Stored in transport media,
- Improperly stored,
- Failure
to meet shipping/transportation
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Additional Information
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N/A
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