WhoopingCoughPCR


Bordetella pertussis, parapertussis, holmesii – PCR

Laboratory Fee Schedule

Procedure:  MZZ0377A 

CPT: N/A


Bordetella pertussis, parapertussis, holmesii – PCR
Synonym(s): Whooping cough
Requisition Form G-2B
Test Description Real-time PCR for B. pertussis, B. parapertussis, B. holmesii
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A  
Performed on Specimens from (sources) Humans
Sample/Specimen Type for Testing
  • Nasopharyngeal swabs
  • Nasopharyngeal washes in 0.85% saline
  • Bacterial isolates
Minimum Volume/Size Required
  • Nasopharyngeal swabs: N/A
  • Nasopharyngeal wash: 200µL
Storage/Preservation Prior to Shipping
  • Must be a rayon or dacron swab with metal or wooden handle with no transport media.
  • Must be maintained between 2-8°C. Do not freeze.
  • Must be received at laboratory within seven (7) days.
Transport Medium
  • N/A
Specimen Labeling
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
  • Must be maintained between 2-8°C. Do not freeze.
  • Must be received on ice packs.
Method
  • Real-time PCR
Turn-around Time 48 business hours from receipt
Interferences/Limitations

Heme from hemoglobin interferes with PCR in this assay.

Common Causes for Rejection
  • Insufficient specimen,
  • Bloody swab,
  • Stored in transport media,
  • Improperly stored,
  • Failure to meet shipping/transportation
Additional Information N/A
   

Last updated August 20, 2020