• Loading...

    Contact Us

    Laboratory Services Section
    MC 1947
    PO Box 149347 Austin, TX 78714-9347
    1100 W. 49th Street
    Austin, TX 78756-3199

    Phone: (512) 776-7318
    Fax: (512) 776-7294

    Phone Us Toll Free at:
    (888) 963-7111, ext. 7318

    Mailing/Shipping Info

    Email the Laboratory

Microbiology Laboratory Tests: Taeniasis to Trichuriasis

Loading...

Microbiology
Taeniasis (Microscopic – Direct Concentration Clearing)
< Return to Table of Contents

 

Test Includes: Microscopic Examination

Reporting

Results Available: 3 days

Contact #s: (512) 458-7560

Reference

Method: Microscopic Examination

 

Turnaround Time:  3 days

Reference Range:  No parasites found

Limitations:

Interpretation:

Specimen Requirements

Specimen Collection: Feces

Sample Type: Feces

Volume/Amount Required: 15 mL liquid stool, 15 g stool,

Preferred Specimen:  Feces in formalin; Proglottid; Scolex

Collection/Preservation: Collect stool in clean, dry container. Immediately transfer to formalin preservative. Hold at ambient temperature.

Storage Instructions: ambient temperature

Causes for Rejection:

Sample Container: Formalin transport for parasites.

Sample Test Kit:

Availability: Monday-Friday

Diagnostic Information:  Fecal specimens must be submitted fresh (less than five hours) or in  formalin.  Submit proglottid in ethyl alcohol.  Referred material from hospital, private, and reference labs.  Examination of proglotids can help to determine species of Taenia infection.

Specimen Submission

Required Request Form: G-1A

Specimen Handling: Handle body fluids using universal precautions. Formalin is a poison, handle with care.

Transport Temperature:  Ambient temperature

Shipping Requirements:  Triple contained in accordance with federal shipping regulations for diagnostic specimens.

Billing

CPT Code: 87015

Fees:

 


Microbiology
Tapeworm, Other Diphyllobothrium latum, Hymenolepis nana, Hymenolepis diminuta 
(Microscopic – Clearing Exam)
< Return to Table of Contents
 
Test Includes: Microscopic Examination

Reporting

Results Available: 3 days Contact #s: (512) 458-7560

Reference

Method: Microscopic Examination  
Turnaround Time:  3 days Reference Range:  No parasites found
Limitations: Interpretation:

Specimen Requirements

Specimen Collection: feces, proglottid, scolex Sample Type: Proglottid; Scolex; Feces
Volume/Amount Required: 15 mL liquid feces, 
15 g feces
Preferred Specimen:  Proglottid; Scolex; Feces
Collection/Preservation: Collect the stool in a clean, dry container. Immediately transfer to formalin. Store at ambient temperature. Collect proglottid or scolex and place in a clean leak-proof container with ethyl alcohol. Storage Instructions: Ambient temperature
Causes for Rejection: Unpreserved stool > 5 hours old Sample Container: Formalin transport for parasites. Clean leak-proof container with ethyl alcohol for transport of proglottid, scolex.
Sample Test Kit: Availability:
Diagnostic Information:  Fecal specimens must be submitted fresh (less than five hours) or in  formalin.  Submit proglottid in ethyl alcohol.  Referred material from hospital, private, and reference labs.

Specimen Submission

Required Request Form: G-2B Specimen Handling: Handle body fluids using universal precautions. Formalin is a poison, handle with care.
Transport Temperature:  Ambient  temperature Shipping Requirements: Triple contained in accordance with federal shipping regulations for diagnostic specimens.

Billing

CPT Code: 87177 Fees:
 
 


Microbiology
Tetanus (Culture – Isolation)
See also Anaerobic Bacterial Culture, Special (Isolation)
< Return to Table of Contents
 

Test Includes: Conventional biochemicals, Rapid ANA, API  Ana Ident, GLC

Reporting

Results Available: 5-10 days

Contact #s: (512) 458-7582

Reference

Method: GLC, Conventional biochemicals, Rapid Ana; API Ana Ident.

Turnaround Time:  5-10 days

Reference Range:  No bacteria isolated

Limitations:

Interpretation:

Specimen Requirements

Specimen Collection: Biopsy

Sample Type: Tissue

Volume/Amount Required: Small piece of tissue

Preferred SpecimenTissue

Collection/Preservation: By biopsy, collect a small piece of tissue. Place in a sterile, leak-proof container.

Storage Instructions: 2-8° C

Causes for Rejection: Aerobic environment used for shipment.

Sample Container: Anaerobic environment

Sample Test Kit:

Availability: Monday-Friday
With prior notification: Saturday-Sunday

Diagnostic Information:  See Anaerobic Bacterial Culture, Special (Isolation)

Specimen Submission

Required Request Form: G-2B

Specimen Handling: Handle body fluids using universal precautions

Transport Temperature:  2-8º C

Shipping Requirements: Triple contained in accordance with federal shipping regulations for infectious agents.

Billing

CPT Code: 87075

Fees:

 

Microbiology
Tetanus (Culture – Identification) See also Anaerobic Bacterial Culture (Identification)

< Return to Table of Contents

 

Test Includes: Conventional biochemicals, Rapid ANA, API  Ana Ident, GLC

Reporting

Results Available: 5-10 days

Contact #s: (512) 458-7582

Reference

Method: GLC, Conventional biochemicals, Rapid Ana; API Ana Ident.

Turnaround Time:  5-10 days

Reference Range: By Report 

Limitations:

Interpretation:

Specimen Requirements

Specimen Collection:

Sample Type: Pure culture

Volume/Amount Required: one specimen per patient

Preferred Specimen:  Pure culture in anaerobic transport medium

Collection/Preservation:

Storage Instructions: ambient temperature

Causes for Rejection: Aerobic transport used for shipment

Sample Container: anaerobic transport

Sample Test Kit:

Availability: Monday-Friday

Diagnostic Information:  See Anaerobic Bacterial Culture (Identification)

Specimen Submission

Required Request Form: G-2B

Specimen Handling: Infectious agent

Transport Temperature: ambient temperature

Shipping Requirements: Triple contained in accordance with federal shipping regulations for infectious agents.

Billing

CPT Code: 87076

Fees:

 

 

Microbiology
Tetanus (Culture – Toxin Selection) See also Anaerobic Bacterial Toxin Detection (Identification)

< Return to Table of Contents

 

Test Includes: Toxin studies

Reporting

Results Available: 3-7 days

Contact #s: (512) 458-7582

Reference

Method: Animal inoculation

 

Turnaround Time:  3-7 days

Reference Range: No toxin demonstrated

Limitations:

Interpretation:

Specimen Requirements

Specimen Collection: 

Sample Type: Pure culture

Volume/Amount Required: One specimen per patient

Preferred Specimen:  Pure culture in anaerobic transport

Collection/Preservation:

Storage Instructions: ambient temperature

Causes for Rejection: Aerobic transport used for shipment.

Sample Container: Anaerobic transport

Sample Test Kit:

Availability: Monday – Friday
With prior notification: Saturday-Sunday

Diagnostic Information:  Anaerobic Bacterial Toxin Detection (Identification)

Specimen Submission

Required Request Form: G-2B

Specimen Handling: Infectious agent

Transport Temperature:  Ambient temperature

Shipping Requirements: Triple contained in accordance with federal shipping regulations for infectious agents.

Billing

CPT Code: 87076

Fees:

 
Microbiology
Toxocariasis (Serological – Forwarded by TDSHS to CDC for testing.)
< Return to Table of Contents
 

Reporting

Results Available: 3 weeks Contact #s:

Reference

Method:  
Turnaround Time:  3 weeks Reference Range:  <1.00 (Nonreactive)
Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.

Specimen Requirements

Specimen Collection: Venipuncture Sample Type: Serum
Volume/Amount Required: 10 mL whole blood Preferred Specimen:  Single Serum
Collection/Preservation: Red top or tiger top tube Storage Instructions: Do not freeze or refrigerate whole blood. Separated serum may be held at 2-8°C
Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing Sample Container: Red top or tiger top tube
Sample Test Kit: Availability:
Diagnostic Information:  Prior notification is requested (512) 458-7760. A detailed patient history is required. Serum specimens are forwarded to the CDC. The EIA procedure uses the excretory-secretory antigen of T. canis larvae to minimize crossreactivity with Ascaris spp and of other parasites. Results must be interpreted with caution, as broad variations in antibody response occur and levels may remain elevated for years after infection. Generally, antibody levels are highest in visceral larvae migrans (VLM). A Nonreactive result usually rules out infection with Toxocara spp.  

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature:  Ambient temperature for specimens on the blood clot, separated serum at 2-8°C (refrigerated) or -20°C (frozen). Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code: 86682 Fees:
 
 


Microbiology
Toxoplasmosis (Serological – Enzyme Immunoassay (IgM))
< Return to Table of Contents
 
Test Includes:

Reporting

Results Available: 3-5 days Contact #s:

Reference

Method: EIA  
Turnaround Time:  3-5 days             Reference Range:  <1.00 (Nonreactive)
Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.

Specimen Requirements

Specimen Collection: Venipuncture Sample Type: Serum
Volume/Amount Required: 2 mL Serum Preferred Specimen:  Single Serum; Paired Sera
Collection/Preservation:
Samples that will be delivered to the laboratory within 8 hours of collection may be transported at room temperature in the original blood collection tube. If the samples are going to be shipped and will be delivered to the laboratory within 48 hours of collection, sera must be separated from the blood and shipped on cold packs, between 2° and 8°C. If the serum samples will not be delivered to the laboratory within 48 hours of collection at these temperatures, then the samples must be frozen at -20°C or lower and shipped on dry ice.
Storage Instructions: Separated serum may be held at 2-8°C, or frozen at -20°C.
Causes for Rejection:
Discrepancy between name on tube and name on form, insufficient quantity of serum for testing, specimens received with extended transit time, or received at incorrect temperature.
Sample Container: Red top or tiger top tube
Sample Test Kit: Availability: Test performed twice per week
Diagnostic Information:  Diagnosis in a newborn requires either a significant rise in toxoplasma IgG level or positive toxoplasma IgM test and IgG titer equivalent to the maternal IgG titer.  For serodiagnosis of acute infection in obstetric patients, titers of 1:64 are usually accepted as minimal for IgM, and titers of 1:1024 are minimal for IgG in adults.  For ocular Toxoplasmosis, titers as low as 1:16 can be significant, however, the disease is diagnosed clinically.  A positive IgM result indicates infection with a high probability the infection occurred within the previous 12 months. In AIDS patients, toxoplasma IgG and IgM antibody levels are low or not detected. Test is performed twice per week.  

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature: Cold (2-8º C) on ice packs if received within 48 hours from time of collection. Frozen (≤-20°) on dry ice if received more than 48 hours from time of collection. Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code: 86778 Fees:
 
 


Microbiology
Toxoplasmosis (Serological – Indirect Fluorescent Antibody (IgG)
< Return to Table of Contents
 
Test Includes:

Reporting

Results Available: 3-5 days Contact #s:

Reference

Method: IFA  
Turnaround Time:  3-5 days Reference Range:  <1:8 (Nonreacative)
Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.

Specimen Requirements

Specimen Collection: Venipuncture Sample Type: Serum
Volume/Amount Required: 2 mL Serum Preferred Specimen:  Single Serum; Paired Sera
Collection/Preservation: Samples that will be delivered to the laboratory within 8 hours of collection may be transported at room temperature in the original blood collection tube. If the samples are going to be shipped and will be delivered to the laboratory within 48 hours of collection, sera must be separated from the blood and shipped on cold packs, between 2° and 8°C. If the serum samples will not be delivered to the laboratory within 48 hours of collection at these temperatures, then the samples must be frozen at -20°C or lower and shipped on dry ice. Storage Instructions: Separated serum may be held at 2-8°C, or frozen at -20°C. 
Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing, specimens received with extended transit time, or received at incorrect temperature. Sample Container: Red top or tiger top tube
Sample Test Kit: Availability: Test performed twice per week
Diagnostic Information:  Diagnosis in a newborn requires either a significant rise in toxoplasma IgG level or positive toxoplasma IgM test and IgG titer equivalent to the maternal IgG titer.  For serodiagnosis of acute infection in obstetric patients, titers of 1:64 are usually accepted as minimal for IgM, and titers of 1:1024 are minimal for IgG in adults.  For ocular Toxoplasmosis, titers as low as 1:16 can be significant, however, the disease is diagnosed clinically.  In AIDS patients, toxoplasma IgG and IgM antibody levels are low or not detected. A single IgG titer is not diagnostic; a four-fold rise in titer between acute and convalescent serum specimens gives meaningful information. The test is performed twice per week.  

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature: Cold (2-8º C) on ice packs if received within 48 hours from time of collection. Frozen (=-20°) on dry ice if received more than 48 hours from time of collection. Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code: 86777 Fees:
 
 


Microbiology
Toxoplasmosis (Microscopic – Giemsa Stain)
< Return to Table of Contents
 
Test Includes: Microscopic examination

Reporting

Results Available: 3 days Contact #s: (512) 458-7560

Reference

Method: Microscopic examination  
Turnaround Time:  3 days Reference Range: No parasites found
Limitations: Interpretation: Negative test results indicate that there were no visible parasites in the specimen submitted.

Specimen Requirements

Specimen Collection: Biopsy Sample Type: Tissue
Volume/Amount Required: small piece of tissue Preferred Specimen:  Tissue
Collection/Preservation: Collect a small piece of tissue by biopsy and transport at ambient temperature. Storage Instructions: ambient temperature
Causes for Rejection: Name on container/form do not match, broken in transport Sample Container: Sterile, leak-proof container
Sample Test Kit: Availability: Monday-Friday
Diagnostic Information:  Serological results are normally used to diagnose toxplasmosis.  Results of all other procedures must be interpreted in light of the serologic findings. T. gondi identified in histological preparations may not be the causative agent of the symptoms (many individuals harbor the organisms in their tissues but have no symptoms).

Specimen Submission

Required Request Form: G-2A Specimen Handling: Handle body fluids and tissues using universal precautions
Transport Temperature:  Ambient temperature Shipping Requirements: Triple contained in accordance with federal shipping regulations for diagnostic specimens.

Billing

CPT Code: 87177 Fees:
 
 


Microbiology
Trachoma (Culture – DNA Probe) See also Chlamydia (Genetic Probe)
< Return to Table of Contents
 
Test Includes: Gen-Probe Pace 2C, Pace 2 for C. trachomatis, AmpCT (amplified)

Reporting

Results Available: 2-3 days Contact #s: (512) 458-7582

Reference

Method: Genetic probe for rRNA
Turnaround Time:  3 days Reference Range:  Negative for C. trachomatis
Limitations: Results dependent on adequacy of sampling. Urine specimens can only be tested using amplified test. Interpretation: Negative results indicate that the patient does not have detectable amounts of C. trachomatis rRNA.

Specimen Requirements

Specimen Collection: Only swabs contained in the PACE specimen collection kit can be use to collect patient specimens. Specimen: female endocervical swab; male urethral swab, conjuctival swab; Urine (amplified only)
Volume/Amount Required: 1 Gen-Probe collector per patient Preferred Specimen:  (nonamplified) Female Endocervical Swab; Male urethral Swab; Conjunctival Swab.
(amplified) Urine
Collection/Preservation: Storage Instructions:
Causes for Rejection: Wrong name on collection tube, Two swabs in tube, No swab on tube, Wooden swab in tube, Metal swab in tube, Insufficient amount of liquid to test, > 7 days old, > 80 uL of blood. Sample Container: Gen-Probe collection tubes
Sample Test Kit: Gen-Probe Pace 2C, Pace 2 CT Availability: Monday-Friday
Diagnostic Information:  

Specimen Submission

Required Request Form: G-2B Specimen Handling:
Transport Temperature: Ambient temperature  Shipping Requirements: In accordance with federal shipping regulations for diagnostic specimens, non-infectious.

Billing

CPT Code:  87490, 87491 Fees:
 

Microbiology
Trachoma (Serological – Forwarded by TDSHS to CDC for testing.)
< Return to Table of Contents

 
Test Includes:

Reporting

Results Available: 3 weeks Contact #s:

Reference

Turnaround Time:  3 weeks Reference Range:  <1:1 (Nonreactive)
Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.

Specimen Requirements

Specimen Collection: Venipuncture Sample Type: Serum
Volume/Amount Required: 10 mL whole blood Preferred Specimen:  Paired Sera
Collection/Preservation: Red top or tiger top tube Storage Instructions: Do not freeze or refrigerate whole blood. Separated serum may be held at 2-8°C
Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing Sample Container: Red top or tiger top tube
Sample Test Kit: Availability:
Diagnostic Information:  Prior notification is requested (512) 458-7760. A detailed patient history is required. See Lymphogranuloma Venereum.  LGV serology available at CDC.  Serology is by prior arrangement only.  Once cleared, serum specimens are sent to the CDC. A four-fold rise in titer between acute and convalescent specimens is indicative of infection  

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature: Ambient temperature for specimens on the blood clot, separated serum at 2-8°C (refrigerated) or -20°C (frozen). Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code: 86631, 86632 Fees:
 
 


Microbiology
Trichinelliasis (Serological – Forwarded by TDSHS to CDC for testing.)
< Return to Table of Contents
 
Test Includes:

Reporting

Results Available: 3 weeks Contact #s:

Reference

Method:  
Turnaround Time:  3 weeks Reference Range:  <1.00 (Nonreactive)
Limitations: May not detect a recent infection, or infection in a person with a severely compromised immune system. Interpretation: Nonreactive indicates that the patient does not have detectable antibody to the infectious agent. Reactive indicates that the patient has detectable antibody to the infectious agent, and depending on the clinical picture, may have a current or past infection.

Specimen Requirements

Specimen Collection: Venipuncture Sample Type: Serum
Volume/Amount Required: 10 mL whole blood Preferred Specimen:  Single Serum
Collection/Preservation: Red top or tiger top tube Storage Instructions: Do not freeze or refrigerate whole blood. Separated serum may be held at 2-8°C
Causes for Rejection: Discrepancy between name on tube and name on form, insufficient quantity of serum for testing Sample Container: Red top or tiger top tube
Sample Test Kit: Availability:
Diagnostic Information:  Prior notification is requested (512) 458-7760. A detailed patient history is required. Serum specimens are forwarded to the CDC. An excretory-secretory antigen is employed to reduce non-specific reactivity; however, cross-reactivity with other parasitic antigen (strongyloides, filarial, malaria) may occur. This cross-reactivity is usually associated with results in the equivocal range.

Specimen Submission

Required Request Form: G-2A Specimen Handling: Use Universal Precautions
Transport Temperature:  Ambient temperature for specimens on the blood clot, separated serum at 2-8°C (refrigerated) or -20°C (frozen). Shipping Requirements: Triple contain, separated serum may be shipped on cold packs (2-8°C), or frozen (-20°C) and mailed on dry ice.

Billing

CPT Code: 86784 Fees:
 
 


Microbiology
Trichinelliasis (Microscopic – Direct)
< Return to Table of Contents
 
Test Includes: Microscopic examination

Reporting

Results Available: Contact #s:

Reference

Method: microscopic examination  
Turnaround Time:  3 weeks Reference Range: No parasites found
Limitations: Interpretation: Negative specimen indicates that there were no visible parasites in the specimen submitted.

Specimen Requirements

Specimen Collection: Biopsy Sample Type: Tissue
Volume/Amount Required: small piece of tissue Preferred Specimen:  Tissue
Collection/Preservation: Collect by biopsy a small piece of muscle tissue. Hold at ambient temperature Storage Instructions: Ambient temperature, do not refrigerate or freeze
Causes for Rejection: Sample Container: Sterile, leak-proof container.
Sample Test Kit: Availability: Monday-Friday
Diagnostic Information:  The suspicion of trichinellosis (trichinosis), based on clinical symptoms and eosinophilia, can be confirmed by specific diagnostic tests, muscle biopsy and microscopy.

Specimen Submission

Required Request Form: G-2B Specimen Handling: Handle body fluids and tissues using universal precautions.
Transport Temperature:  Ambient  temperature Shipping Requirements: Triple contained in accordance with federal shipping regulations for diagnostic specimens.

Billing

CPT Code: 87177 Fees:
 
 


Microbiology
Trichuriasis (Microscopic – Direct Concentration Exam ID)
< Return to Table of Contents
 

Test Includes: Microscopic examination

Reporting

Results Available: 3 days

Contact #s: (512) 458-7560

Reference

Method: Microscopic examination

 

Turnaround Time:  3 days

Reference Range:  No parasites found

Limitations:

Interpretation:  Negative specimen indicates that there were no visible parasites in the specimen submitted.

Specimen Requirements

Specimen Collection: Feces

Sample Type: Feces, worms

Volume/Amount Required: 15 mL liquid stool, or 15 g stool

Preferred Specimen:  Feces; Worm

Collection/Preservation: Collect stool in a clean, dry container. Immediately transport to formalin preservative.

Storage Instructions: Formalin preservative

Causes for Rejection: Unpreserved stool > 5 hours old.

Sample Container: Formalin transport for parasites

Sample Test Kit:

Availability: Monday-Friday

Diagnostic Information:  Fecal specimens must be sent in fresh (less that five hours) and in formalin. Adult worm can be submitted for identification in ETOH or in 10 % formalin.  Referred material accepted from hospital, private, and reference labs.

Specimen Submission

Required Request Form: G-2B

Specimen Handling: Handle body fluids using universal precautions. Formalin is a poison, handle with care.

Transport Temperature:  Ambient temperature

Shipping Requirements: Triple contained in accordance with federal shipping regulations for diagnostic specimens.

Billing

CPT Code: 87015

Fees:

 
 
Last updated September 17, 2010