Test Includes: Nucleic Acid Amplification Testing of Clinical Specimens for Mycobacterium tuberculosis complex |
Reporting
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Results Available: 48 hours |
Contact #s: 512-776-7342 or 512-776-7580 |
Reference
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Method: Cepheid GeneXpert PCR test amplifies and detects DNA of M. tuberculosis complex |
Turnaround Time: 48 hours from specimen receipt in this laboratory. Positive reports are communicated to the specimen submitter on day of testing. |
Reference Range: Normal value = Negative |
Limitations: Specimen must be sputum, induced or expectorated. AFB Smear and Culture always performed concurrently at this laboratory. A negative test does not mean the patient does not have M. tuberculosis disease.Test results must be confirmed by culture of specimen for the presence of mycobacteria. If a processed sputum sediment is submitted for NAAT only, the AFB culture must be in progress at the submitting laboratory site. |
Interpretation: A Positive result means that the DNA of the M. tuberculosis complex is detected. A Negative result means that the DNA of the M. tuberculosis complex was not detected. A Negative result does not rule out the possibility of the presence of viable M. tuberculosis complex in the specimen. |
Specimen Requirements
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Specimen Collection: Follow the Guidelines for Specimen Collection and Submission: Mycobacteriology (AFB) Collection, Transport, and Storage. |
Sample Type: Clinical |
Volume/Amount Required: 3 mL to 15 mL |
Preferred Specimen: Sputum or processed sputum sediment. For all other specimen types, please contact 512-776-7342 or 512-776-7580. |
Collection/Preservation: No preservative |
Storage Instructions: Transport specimen as soon as possible. Diagnostic specimens should be shipped on cold pack overnight. |
Causes for Rejection: Specimen leaked in transit. No identifier on specimen container. No test requested on G-MYCO requisition form. |
Sample Container: Triple contained. Sterile, leak-proof, 50 mL conical tube preferred for primary container. |
Sample Test Kit: |
Availability: Monday through Friday |
Diagnostic Information: There should be a high clinical suspicion of tuberculosis when ordering this test. NAA testing should be requested if the test results will make a difference to the patient’s treatment or if the test results will make a difference to the contact investigation. Patients should not have a laboratory diagnosis of tuberculosis disease already. |
Specimen Submission
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Required Request Form: G-MYCO
- For sputum specimens, check test “AFB Smear, Culture and Direct NAAT (Respiratory Diagnostic Specimens Only)"
- For processed sputum sediments, check test “Direct NAAT for M. tuberculosis”
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Specimen Handling: Follow the Guidelines for Specimen Collection and Submission: Mycobacteriology (AFB) Collection, Transport, and Storage. |
Transport Temperature: Cold shipment (not frozen) recommended. |
Shipping Requirements: Triple-contained and packaged to meet requirements of DOT, USPS, PHS, and IATA for shipping of clinical specimens. Please check with your courier. |
Billing
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CPT Code: 87556 |
Fees: http://www.dshs.state.tx.us/lab/fees.shtm |