Bacillus anthracis PCR

Bacillus anthracis PCR

Laboratory Fee Schedule

Procedure #:  BTA0024

CPT:  87798


Synonym(s): Bacillus anthracis PCR, Anthrax PCR
Requisition Form G-27A
Test Description Detection of B. anthracis DNA by PCR
Pre-Approval Needed Must notify BioThreat Team prior to sample submission:  (512) 689-5537
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Non-Human (Bacterial), Human, Environmental
Sample/Specimen Type for Testing Culture isolates, whole blood (EDTA or sodium citrate), serum, plasma, pleural fluid, respiratory specimens (trastracheal aspirates, bronchial lavage), CSF, sputum, clinical swabs, environmental (swabs, powders, liquids)
Minimum Volume/Size Required Culture isolates 1 plate or slant, clinical samples 1 mL
Storage/Preservation Prior to Shipping

Ambient:

•   Culture isolates, clinical or environmental samples

2-8°C (cold):

•   Clinical samples

•   Environmental samples

Transport Medium N/A
Specimen Labeling

Human

•   At least 2 patient specific identifiers: First and last name as one identifier and a DOB or a

    unique patient specific identifier (e.g. Medical record number).

•   Specimen identification must match submission form.

Non-Human or Environmental

•   Sample identification must match submission form.

Shipping and Specimen Handling Requirements

•   Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.

•   Handled as infectious agent using universal precautions.

•   Triple contained in accordance with federal shipping regulations for infectious agents.

•   See: Emergency Preparedness resources online.

•   Notify BioThreat Team prior to sample submission: (512) 689-5537

Method PCR
Turn-around Time Preliminary result 1 day after receipt, final result 3-7 days
Interferences/Limitations Improper storage or shipping temperatures may limit organism recovery.
Common Causes for Rejection

•   Insufficient quantity

•   Unacceptable specimen type or source

•   Improper shipping conditions

•   Expired media or collection container

•   Discrepancies between specimen label and submission form

•   Incomplete or missing submission form

Additional Information May be paired with Definitive ID: Bacillus anthracis


Last updated January 14, 2019