BurkholderiaPCR

PCR: Burkholderia mallei/pseudomallei

Laboratory Fee Schedule

Procedure #:  BTA0026

CPT:  87798

PCR: Burkholderia mallei/pseudomallei

Synonym(s):

Melioidosis or Burkholdeia pseudomallei, glanders or Burkholderia mallei PCR

Requisition Form

G-27A

Test Description

Detection of Burkholderia mallei/pseudomallei DNA by PCR

Pre-Approval Needed

Must notify BioThreat Team prior to sample submission: (512) 689-5537

Supplemental Information Required

N/A

Supplemental Form(s)

N/A

Performed on Specimens from (sources)

Non-Human (Bacterial), Human, Environmental

Sample/Specimen Type for Testing

Culture isolates, whole blood, serum, environmental specimens

Minimum Volume/Size Required

Culture isolates 1 plate or slant, clinical samples 1 mL

Storage/Preservation Prior to Shipping

Culture isolates ambient, clinical or environmental samples 2-8 °C (cold)

Transport Medium

N/A

Specimen Labeling

Human

•   At least 2 patient specific identifiers: First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. Medical record number).

•   Specimen identification must match submission form.


Non-Human or Environmental


•   Sample identification must match submission form.

Shipping and Specimen Handling Requirements

•   Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.

•   Handled as infectious agent using universal precautions.

•   Triple contained in accordance with federal shipping regulations for infectious agents.

•   See also Emergency Preparedness resources online.

•   Notify BioThreat Team prior to sample submission: (512) 689-5537

Method

PCR

Turn-around Time

Preliminary result 1 day after receipt, final result 4-21 days

Interferences/Limitations

Improper storage or shipping temperatures may limit organism recovery.

Causes for Rejection

Insufficient quantity, unacceptable specimen type or source, improper shipping conditions, expired media or collection container, discrepancies between specimen label and submission form, and/or incomplete or missing submission form

Additional Information

May be paired with Definitive ID – Burkholderia mallei/pseudomallei

Last updated November 5, 2018