Definitive ID:  Burkholderia mallei/pseudomallei

Laboratory Fee Schedule

Procedure #:  BTA0002

CPT:  87040, 87070, 87077

Definitive ID: Burkholderia mallei/pseudomallei
Synonym(s): Melioidosis or Burkholdeia pseudomallei, Glanders or Burkholderia mallei
Requisition Form G-27A
Test Description Culture identification of Burkholderia mallei/pseudomallei
Pre-Approval Needed Must notify BioThreat Team prior to sample submission: (512) 689-5537
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Non-Human (Bacterial), Human
Sample/Specimen Type for Testing Pure culture isolates, bone marrow, whole blood, sputum, wound swabs, tissue, urine
Minimum Volume/Size Required Culture isolates 1 plate or slant
Storage/Preservation Prior to Shipping Ambient
Transport Medium N/A
Specimen Labeling


•   At least 2 patient specific identifiers: First and last name as one identifier and a DOB or a unique patient specific identifier (e.g. Medical record number).

•   Specimen identification must match submission form.


•   Sample identification must match submission form.

Shipping and Specimen Handling Requirements

•   Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.

•   Handled as infectious agent using universal precautions.

•   Triple contained in accordance with federal shipping regulations for infectious agents. 

•   See also Emergency Preparedness resources online.

•   Notify BioThreat Team prior to sample submission:  (512) 689-5537

Method Conventional biochemical methods
Turn-around Time Preliminary result 1 day after receipt, final result 4-21 days
Interferences/Limitations Use dacron or rayon swabs for sample collection, cotton swabs interfere with PCR.  Material must be viable.  Improper storage or shipping temperatures may limit organism recovery.
Causes for Rejection Insufficient quantity, unacceptable specimen type or source, improper shipping conditions, expired media or collection container, discrepancies between specimen label and submission form, and/or incomplete or missing submission form
Additional Information Will be paired with PCR: Burkholderia mallei/ pseudomallei

Last updated November 5, 2018